Lentiviruses (pLKO.1 and pGIPZ viruses) and AAVs were prepared following our established protocols (5052), and their titers were estimated by quantitative PCR (qPCR)–based methods (50, 51, 53). For lentiviruses, pLKO.1-Gstm1 shRNA lentiviral vectors (#1, TRCN0000103241; #2, TRCN0000103243; #3, TRCN0000103244; and #4, TRCN0000103240) were obtained from the RNAi Consortium (TRC) library via the HiT Center at the Johns Hopkins University School of Medicine; control pLKO.1-GFP shRNA lentiviral vector (no. 30323) was obtained from Addgene, and pGIPZ-Gstt2 shRNAmir lentiviral vectors (#1, V2LMM_67055; #2, V2LMM_218573; #3, V3LMM_449685; and #4, V3LMM_449688) and control nonsilencing (NS) shRNAmir lentiviral vector (RHS4346) were obtained from Open Biosystems. pHAGE-Gstm1 and pHAGE-Gstt2 were generated by subcloning mouse Gstm1 and Gstt2 complementary DNAs (cDNAs) into pHAGE vectors. For AAVs, AAV-LSL-GFP-Gstm1 shRNAmir was generated on the basis of the most efficient shRNA construct (#4) (fig. S2), following the established protocol (54), and AAV-LSL-GFP-Gstt2 shRNAmir and AAV-LSL-GFP-NS shRNAmir were generated by shuttling Gstt2-shRNAmir (#2) and NS-shRNAmir from pGIPZ lentiviral vectors into AAV-LSL-GFP vectors.

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