TCRα mice were purchased from the Jackson Laboratory (87). BMPR1αT− mice were generated by crossing BMPR1α conditional knockout mice with mice expressing CD4cre and Foxp3GFP reporter (25, 88, 89). Foxp3GFP and BMPR1αT− mice were crossed with mice expressing TCR specific for analog of peptide derived from PCC (90). All mice were on the C57BL6 genetic background. Mice were bred and housed under specific pathogen–free conditions in the animal facility of Old Dominion University (ODU). All experiments were approved by Institutional Animal Care and Use Committee. Both female and male mice were used in experiments, and we have not observed any difference in T cell development and activation between sexes. Mice were 6 to 12 weeks old for all experiments.

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