The full length of Lgl open reading frame was amplified by polymerase chain reaction (PCR) with the addition of the Xho I and Pme I restriction sites at the N and C terminus, respectively. The primers used were as follows: Lgl–Xho I, 5′-ACGTCTCGAGCAACATGTTAAAGTTTATC-3′; Lgl–Pme I, 5′-ACGTGTTTAAACGCAAATTGGCTTTCTTC-3′. The PCR products were digested and inserted into the Xho I/Eco RV sites of the pMK33-C-SBP vector.

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