Statistical analyses were performed using Student’s t test (paired or unpaired), Mann-Whitney rank sum test, Wilcoxon signed-rank test (in experiments where samples failed normality), and ANOVA as indicated. Post hoc testing on ANOVA was done by multiple pairwise comparison procedures using the Holm-Sidak method. Statistical analysis was performed using SigmaPlot 13.0 (Systat Software Inc.). Outliers were determined by Grubb’s test (GraphPad Software). P value of less than or equal to 0.05 was defined as statistically significant. The correlation between CD8+ T cell infiltration and inhibition of Y-COM was analyzed by Spearman’s rank-order correlation.

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