The Mann-Whitney was used to compare the fibroblasts from WFS1 patients and controls. The Kruskal-Wallis test was used to compare the effect of the overexpression of NCS1 or vector alone. Differences were considered significant at *P < 0.05, **P < 0.01, and ***P < 0.001. Proportions of ER-mitochondrial contacts between controls and patient fibroblasts were compared with their 95% confidence intervals.

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