Activated CD8+ T cells were stimulated with 1 μM CGS21680 for 30 min. Cell lysates were prepared as previously described, and total protein content was measured using BCA Protein Assay (55). The cell lysates were diluted to equal protein concentrations using kinase activity assay buffer. The PKA kinase activity was measured using a PKA kinase activity kit (Enzo Life Sciences) as described by the manufacturer, with a reaction time of 90 min at 30°C and a development time of 60 min. The relative kinase activity was determined by the absorbance of the sample at 450 nm divided by the amount (micrograms) of crude protein.

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