Activated CD8+ T cells were lysed using 0.1 M HCl at a final concentration of 1 × 106 cells/ml. Intracellular cAMP concentrations were measured in T cell lysates using the acetylated procedure of the Direct cAMP ELISA Kit (Enzo Life Sciences) according to the manufacturer’s instructions.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.