All confocal experiments were performed on fibroblasts placed on the stage of a Zeiss LSM 510 inverted confocal microscope (Zeiss, Le Pecq, France). Pericam-mt/pcDNA was used to measure [Ca2+]m. To measure cytosolic Ca2+, fibroblasts were loaded with Fluo-4 AM (5 μM; Molecular Probes), Fluo-4 signal was obtained by excitation at 488 nm, and emitted light was collected at 515 nm. For pericam-mt, the excitation wavelength upon Ca2+ binding switches from 415 to 488 nm. Thus, the transfected cells were excited at 488, and images were collected at 515 nm. All the collected images were processed with ImageJ (NIH, USA; http://rsb.info.nih.gov/ij/). All confocal experiments were performed on fibroblasts placed on the stage of a Zeiss LSM 510 inverted confocal microscope (Zeiss) equipped with a 63× lens [oil immersion; numerical aperture (NA), 1.2].

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