Mice were sensitized by topical application of 50 μl of 0.5% DNFB (Sigma-Aldrich) in 4:1 acetone/olive oil on their shaved back and were challenged on their ears 4 days later with 10 μl of 0.3% DNFB or vehicle control. Then, 4, 24, and 48 hours later, 10 μl of either vehicle, VUF10661 (50 μM), or VUF11418 (50 μM) dissolved in 72:18:10 acetone:olive oil:DMSO was topically applied to the indicated ear by an investigator blinded to treatment. Ear thickness was measured at different time points with an engineer’s micrometer (Standard Gage).

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