Mice were sensitized by topical application of 50 μl of 0.5% DNFB (Sigma-Aldrich) in 4:1 acetone/olive oil on their shaved back and were challenged on their ears 4 days later with 10 μl of 0.3% DNFB or vehicle control. Then, 4, 24, and 48 hours later, 10 μl of either vehicle, VUF10661 (50 μM), or VUF11418 (50 μM) dissolved in 72:18:10 acetone:olive oil:DMSO was topically applied to the indicated ear by an investigator blinded to treatment. Ear thickness was measured at different time points with an engineer’s micrometer (Standard Gage).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.