Jurkat cells (an immortalized human CD4+ T cell line) stably expressing CXCR3 were generated by transfecting a linearized pcDNA3.1 expression vector encoding geneticin (G-418) resistance, selecting for transfected cells with geneticin (1000 μg/ml), and collecting cells that highly expressed CXCR3 by fluorescence-activated cell sorting (FACS). Cells were maintained in RPMI 1640 medium (Sigma-Aldrich) supplemented with 10% FBS, 1% penicillin/streptomycin, 0.23% glucose, 10 mM Hepes, 1 mM sodium pyruvate, and geneticin (250 μg/ml).

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