The external solution for whole-cell recordings was a Ca2+-containing and Ca2+-free physiological salt solution (CaPSS and Ca2+-free external solution; see solutions I and II, Table 1). The pipette solutions (solutions III, IV, V, and VI) for these experiments were described in Table 1. Thapsigargin, NPPB, IP3, and 2-APB were purchased from Sigma-Aldrich. CPA was purchased from Tocris Bioscience. 2,6-Difluoro-N-(1-(4-hydroxy-1-(trifluoromethyl)benzyl)-1H-pyrazol-3-yl)benzamide (GSK-7975A) was purchased from Aobious. Stock solutions were made by dissolving NPPB, CPA, thapsigargin, and GSK-7975A in dimethyl sulfoxide (DMSO), and IP3 was dissolved in water. Then, these chemicals were diluted in the bath solution to the specific concentrations. Final concentrations of DMSO were <0.1%.

The CC2 peptide and scrambled CC2 peptide were synthesized by NEO BioScience. The sequences for CC2 peptide and scrambled CC2 peptide are provided in Fig. 2F. CC2 peptide and scrambled CC2 peptide were dissolved in pipette solutions (solution IV or VI) for cell dialysis, and final concentrations were 10 μM.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.