IFN-γ recombinant human (no. PHC4033) and mouse (no. PMC4033) proteins were from Gibco (Life Technologies). MRT68921 dual autophagy kinase ULK1/2 inhibitor was purchased from Selleckchem (no. S7949). CQ was purchased from Sigma-Aldrich. XMD8-92 was purchased from Tocris (no. 4132). The Edit-R human lentiviral ULK1 sgRNAs (no. VSGH10142-246477203) and Edit-R Lentiviral hEF1α-Blast-Cas9 nuclease plasmid DNA (no. CAS10138) were purchased from GE Healthcare Dharmacon. ON-TARGETplus Non-targeting Control Pool siRNA (no. D-001810-10-05), ON-TARGETplus PIK3C3 siRNA (SMARTpool: no. L-005250-00-0005), and ON-TARGETplus BECN1 siRNA (SMARTpool: no. L-010552-00-0005) were purchased from GE Healthcare Dharmacon. Antibodies against ULK1 (D8H5; no. 8054), MLK3 (no. 2817), phospho-ERK5 (Thr218/Tyr220; no. 3371), ERK5 (D3I5V; no. 12950), phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (D13.14.4E; no. 4370), p44/42 MAPK (ERK1/2; no. 9102), phospho-SAPK/JNK (Thr183/Tyr185) (81E11; no. 4668), SAPK/JNK (no. 9252), phospho-STAT1 (Ser727) (D3B7; no. 8826), phospho-STAT1 (Tyr701) (D4A7; no. 7649), phospho-p90RSK (Thr359/Ser363) (no. 9344), and RSK1 (D6D5) (no. 8408) were purchased from Cell Signaling Technology. Anti-MLK3 (phospho Thr277 + Ser281) antibody (no. ab191530) was purchased from Abcam, and STAT1 p84/p91 antibody (E-23; no. sc-346) was from Santa Cruz Biotechnology. Antibody against GAPDH (6C5) (no. MAB374) was purchased from EMD Millipore.

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