For each experiment, cells from three SILAC conditions (fig. S1C) were lysed in immunoprecipitation lysis buffer at 4°C. ALK was immunoprecipitated from 8 mg of lysate in parallel for each SILAC condition, and the immunoprecipitated eluates were combined before SDS-PAGE, Coomassie staining, and in-gel digestion essentially as described in (29).

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