NB1, SH-SY5Y, CLBGA, NBL-S, and H3122 cells were cultured in complete medium and treated for the indicated time points with inhibitor (TAE684, LDK378, crizotinib, lorlatinib, LY294002, or U0126) as indicated, with their respective IC50 values reported. Control cells were treated with similar amount of DMSO as the treated cultures (0.1% DMSO). IGF-1 and insulin stimulations were performed using 100 ng/ml for 10 min after ALK inhibitor pretreatment. Cell lysates, protein concentration determination, and Western blotting were carried out as previously described (29).

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