NB1, SH-SY5Y, CLBGA, NBL-S, and H3122 cells were cultured in complete medium and treated for the indicated time points with inhibitor (TAE684, LDK378, crizotinib, lorlatinib, LY294002, or U0126) as indicated, with their respective IC50 values reported. Control cells were treated with similar amount of DMSO as the treated cultures (0.1% DMSO). IGF-1 and insulin stimulations were performed using 100 ng/ml for 10 min after ALK inhibitor pretreatment. Cell lysates, protein concentration determination, and Western blotting were carried out as previously described (29).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.