Cells were fixed with 4% paraformaldehyde for 10 min, stained with 1% Alcian blue 8GX (Sigma-Aldrich), thoroughly washed with distilled water, and mounted in glycerol. Brightfield images of Alcian blue samples (Fig. 5E and fig. S1A) were taken using the Olympus microscope IX71. Phase-contrast images were taken using a Carl ZEISS Axio Observer microscope. The contrast, brightness, and gamma were adjusted.

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