Operant vapor self-administration was conducted in eight airtight chambers (14 cm × 20 cm × 23 cm; La Jolla Alcohol Research, La Jolla, CA, USA; fig. S1) that were placed inside a black Plexiglas enclosure to minimize noise and light. Two nosepoke holes were mounted opposite to each other on the side walls. White light bulbs were mounted above the nosepoke holes. A vacuum pump maintained constant ambient airflow in the chambers. The outflowing air was filtered by an inline HEPA-Cap disposable filter and then disposed through the facility’s exhaust system. To vaporize the drug, we used a vaporizing tank that was equipped with an atomizer (SMOK TFV8 X-Baby Tank; Shenzhen IVPS Technology, Shenzhen, China), which was filled with fentanyl or vehicle solutions. The atomizer was activated by an SVS250 vaporizer (Scientific Vapor, OR, USA). We used Med Associates software and interface (St. Albans, VT, USA) to record nosepokes and control activation of the vaporizers and light cue presentation. The suction system allowed the flow of vaporized drug into the operant chamber when the vaporizer was activated. The duration of drug vapor in the chamber depends on airflow rate, power setting of the vaporizer, and vaporizing time. In our experiments, these were adjusted to allow drug clearance within 1 min after each vapor delivery (verified by visual inspection). In most of the experiments, we used an airflow rate of 1 to 2 liters/min, a power of 60 W, and a vaporizing duration of 1.5 s.

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Mao Ye
Salk Institute 
Hi, it looks very useful for addiction study. I would like to know how to set it up in the lab to study addiction.
2020-10-23 20:30:15 Reply



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