HeLa cells were treated with control siRNA or specific siRNA targeting RPA1 3′ untranslated region 24 hours before cells were transfected with the indicated FLAG-tagged expression constructs. Forty hours after transfection, cells were treated with or without 1 μM CPT for 8 hours. Same numbers of cells for each group were seeded into six-well plate, and the above treatment was repeated twice (every 2 days). Cells were allowed to grow into colonies for a total of 14 days, fixed with 4% (w/v) paraformaldehyde for 10 min, and stained with 0.5% crystal violet for 20 min. The number of colonies per well was counted, and the planting efficiency and surviving fraction for given treatments were calculated on the basis of the survival rates of control cells. Each experiment was performed in triplicate and repeated at least three times.

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