CDYL was fused to GST, expressed in bacteria, and purified using standard protocols. In vitro transcription and translation experiments were done with rabbit reticulocyte lysate (TNT Systems, Promega) according to the manufacturer’s recommendation. The glutathione beads were incubated with the GST-CDYL and in vitro transcribed/translated RPA1 in the buffer containing 10 mM Na-Hepes (pH 7.5), 150 mM NaCl, 0.005% Tween 20, and 2 mM DTT and then were washed in the buffers containing 10 mM Na-Hepes (pH 7.5), 150 to 300 mM KCl, 0.5 to 1.0% Tween 20, and 2 mM DTT. Unless stated elsewhere, 20% of the total protein was used as input.

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