Tryptic peptides were dissolved in NETN buffer [100 mM NaCl, 1 mM EDTA, 50 mM tris-HCl, 0.5% NP-40 (pH 8.0)] and then incubated with antibody beads (PTM Bio Inc., Hangzhou) at a ratio of 15-μl beads per milligram of protein at 4°C overnight. The antibody beads were washed four times with NETN buffer and twice with double-distilled H2O. The Kcr peptides were then eluted by adding elution buffer with 0.1% trifluoroacetic acid. The eluted peptides were cleaned with C18 ZipTips (Millipore) according to the manufacturer’s instructions before being subjected to LC-MS/MS analysis.

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