Cells were harvested and washed twice with ice-cold phosphate-buffered saline (PBS) before they were lysed in NETN buffer (100 mM NaCl, 50 mM tris-HCl, 1 mM EDTA, 0.5% NP-40) supplemented with protease inhibitor cocktail (Protease Inhibitor Cocktail Set III; Calbiochem) and histone deacetylase (HDAC) inhibitor [50 mM nicotinamide (NAM) and 3 μM trichostatin A (TSA)] on ice for 30 min followed by centrifugation at 1000g for 10 min at 4°C. The supernatant was discarded, and the pellet was collected as nuclear fraction samples.

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