HeLa cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (HyClone) supplemented with 10% fetal bovine serum (FBS). All transfections were carried out using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s recommendations. Cells were transfected with siRNA oligonucleotides using Lipofectamine RNAiMAX (Invitrogen) with the final concentration at 25 nM.

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