The complementary DNA (cDNA) for WT RPA1 was amplified by polymerase chain reaction and ligated into pcDNA3.1(−) plasmid containing 3×FLAG tag. RPA1 mutants including K88R, K379R, K595R, and 3KR were generated using the QuikChange Lightning Site-Directed Mutagenesis Kit. The siRNAs were purchased from the GenePharma Company of Shanghai. The target sequences were as follows: RPA1 siRNA, 5′-UUAUCAUCAAGCAGGAAUUAU-3′; CDYL siRNA, 5′-CAGAGAAUAACUCACUAAATT-3′.

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