For SDS-PAGE, indicated cells were lysed in lysis buffer described above. Forty micrograms of whole-cell lysates (or 20 μg for purified mitochondrial lysates) was then loaded onto the SDS–polyacrylamide gel and subjected to electrophoresis. After electrophoresis, gels were transferred to polyvinylidene difluoride membrane and subjected to immunoblotting with indicated antibodies.

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