For nuclear magnetic resonance (NMR) analysis, PBS buffer was prepared in D2O (PBS-d). nIRHT (396 μl; 10 mg/liter) in PBS-d was mixed with 4 μl of 10 mM 5-HT in D2O. The mixture was excited with a 721-nm laser for 10 min on the same nIR fluorescence spectrometer used for nanosensor screening and fluorescence measurements. The solution was spin-filtered using a 100-kDa MWCO centrifugal filter at 6000 rpm for 5 min with D2O, and the flow-through solution was collected. The 1H NMR spectra of the flow-through solution were measured with a 400-MHz Avance AVB-400 NMR (Bruker). The 1H NMR spectra of a 0.1 mM 5-HT solution without laser excitation in D2O were also measured as a control.

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