Jurkat and Molt-4 cells were acquired from the Leibniz Institute DSMZ. We thank B. Ingelsson, O. Stål, and J. Ernerudh for providing human embryonic kidney (HEK) 293T, MCF-7, MDA-MB-231, and HTR-8/SVneo cells. All cell lines were kept in tissue culture–treated flasks in a humidified incubator at 37°C with 5% CO2. HEK293T cells were cultured in high-glucose Dulbecco’s modified Eagle medium (DMEM) (Gibco Thermo Fisher Scientific, no. 41965), MCF-7, and MDA-MB-231 in standard DMEM (Gibco Thermo Fisher Scientific, no. 31053), and HTR-8/SVneo, Jurkat, and Molt-4 cells were kept in the American Type Culture Collection–modified RPMI 1640 medium (Gibco Thermo Fisher Scientific, no. A10491). All culture media were supplemented with 10% fetal bovine serum (FBS) (Gibco Thermo Fisher Scientific, no. 10500) and 1% penicillin-streptomycin [penicillin (100 U/ml) and streptomycin (100 μg/ml)] (Gibco Thermo Fisher Scientific, no. 15140). Cells were passaged every 2 to 3 days during which adherent cells were detached with trypsin (Gibco Thermo Fisher Scientific, no. 15400). For decontamination, cells infected with Mycoplasma were treated with indicated concentrations of Plasmocin (InvivoGen, ant-mpt) for up to 7 days. Every 2 to 3 days, when cells were passaged, fresh antibiotic was added.

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