Adult zebrafish were maintained under a light-dark cycle (14 hours of light and 10 hours of darkness) at 28.5°C. Zebrafish embryos were maintained in a 10-mm petri dish with E3 water at 28.5°C up to 7 dpf. All animal experiments were approved by the Mayo Clinic College of Medicine Institutional Animal Care and Use Committee (protocol A00002783-17).

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