Beads derived from ChIP were resuspended in 11 μl of Laemmli Buffer (4×) and boiled for 30 min at 95°C before dilution with water. Western blot for TDP-43 protein was performed using the mouse anti-rat TDP-43 (Abcam, no. 104223) and donkey anti-mouse IgG IRDye 800CW (Licor).

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