Bioinformatic screening
Animal and human samples
Cell culture and transfections
Plasmids, shRNAs, and DNA cloning
Virus preparation
SV exo-endocytosis evaluation and calcium imaging
Pharmacology
Neurite elongation assay
In utero electroporation
Single-molecule RNA in situ hybridization and immunofluorescence
Quantitative real-time PCR
Library preparation and sequencing
RNA-sequencing analysis
ChIRP DNA analysis
GO and STRING analyses
5′-RACE PCR
ChIRP and RNA interactome analysis
MS identification of protein interactors following ChIRP/RNA interactome analysis
RNA immunoprecipitation
Immunocytochemistry
Statistical analysis and graph construction
Beads derived from ChIP were resuspended in 11 μl of Laemmli Buffer (4×) and boiled for 30 min at 95°C before dilution with water. Western blot for TDP-43 protein was performed using the mouse anti-rat TDP-43 (Abcam, no. 104223) and donkey anti-mouse IgG IRDye 800CW (Licor).