For rapid amplification of 5′-cDNA ends, the GeneRacer Kit with SuperScript III RT and the TOPO TA Cloning Kit for Sequencing (Invitrogen) were used. The gene-specific primers (data S1J) were designed for the 5′ end amplification, and nested PCR products were cloned and sequenced for analyses as reported in data S1C.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.