Primary hippocampal neuron cultures were transfected at 10 DIVs with either a pool of three shRNAs or the pBI construct to down-regulate or overexpress neuroLNC, respectively. A total of >20 neurons per condition were analyzed following 4 days of expression, from three independent cultures. Neurons were manually traced in a blind experimental design and analyzed with the ImageJ plugin NeuronJ. The aspects considered were the average length and the number of primary, secondary, and tertiary neurites (arising respectively from the cell body, from a primary neurite, or from a secondary neurite).

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