For the pharmacological modulation of neuronal activity, we used 1 μM TTX (Na+ Channel blocker; Cayman Chemical, USA), 20 μM bicuculline methobromide (GABAA receptor antagonist; Tocris Bioscience, Cambridge, UK), 100 μM 4-AP (K+ channel blocker; Tocris Bioscience, Cambridge, UK), 10 μM CNQX (AMPA/kainate receptor antagonist; Tocris Bioscience, Cambridge, UK), and 50 μM D-AP5 (N-methyl-d-aspartate receptor antagonist; Tocris Bioscience, Cambridge, UK). A total of >200 neurons from three different cultures were blindly analyzed for each condition.

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