Recombinant AAV particles were generated as described before (41). Briefly, vectors were propagated in HEK293T cells using the pDP6 helper plasmid (avoiding adenoviral contamination). Viral particles were purified by iodixanol step gradient centrifugation followed by heparin affinity chromatography. Fast protein liquid chromatography–purified/concentrated vectors were desalted by dialysis against phosphate-buffered saline (PBS). Viral titer was adjusted by serial dilution on primary hippocampal neurons by monitoring GFP expression levels (always expressed in a second cassette of the construct, driven by the human synapsin-1 promoter).

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