Serial dilution assays for checking DNA damage sensitivity were performed as described in a previous study (37). Cells were grown to OD600 = 1.0 to 2.0 in YPD media and adjusted to OD600 = 0.75 with distilled water for the first dilution. Four 10-fold serial dilutions were prepared by using the first dilution sample. For MMS sensitivity test, 3 μl of five serial dilution samples was subsequently spotted on media plates in the absence or presence of 0.01% MMS and incubated at 30°C for 3 days.

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