TM were imaged with MRI using an alternative protocol to permit interstitial tumor transport analysis. For these animals, a three-dimensional T1-weighted MRI was acquired immediately before the injection of the contrast agent to obtain the baseline signal intensity in the tissue. The contrast agent was then intravenously injected, and a series of four T1-weighted contrast-enhanced MRIS were obtained. Pulse-sequence parameters for all T1-weighted Is were identical: TR/TE, 12/4.35 ms; flip angle, 25°; readout bandwidth, 300 Hz/Px; FOV, 38 by 77 by 36 mm; resolution, 0.3 mm isotropic; and total time per image, 3:04. Following FUS BTB/BBB opening (described previously), a T1-weighted MRI was again acquired to obtain baseline signal intensity for post-FUS measurements. The contrast agent was injected intravenously, and a second series of four T1-weighted contrast-enhanced MRIs was acquired.

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