To prepare a cell-free collagen I master mix of a defined volume (90 μl), 10 μl of a 10× concentrated minimal essential Eagle’s medium (MEM) (M0275, Sigma-Aldrich) was added to 5 μl of a 7.5% sodium bicarbonate (BIC) solution (25080-060, Gibco) in a sterile tube and gently mixed until the indicator color changed from yellow (acidic) to purple (basic). Subsequently, 75 μl of purified bovine collagen I stock solution (3.0 mg/ml; PureCol) (5005-C, Advanced BioMatrix Inc.) was added to the MEM/BIC solution and gently mixed until a homogeneous, slightly purple color was obtained. The collagen I master mix was kept on ice for immediate use.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.