Cell proliferation was measured over a period of 4 days after seeding cells at an initial density of 2600 cells/cm2. MCF10A NLS copGFP proliferation was continuously monitored by a custom epifluorescence microscope housed in a standard tissue culture incubator (37°C, 90% humidity, 5% CO2; 10× objective; 1-hour acquisition intervals). Cell numbers were quantified manually every 24 hours using ImageJ. To measure cell viability, cells were seeded at a density of 1300 cells/cm2 on fibronectin-functionalized PA and silicone gels in a 12-well plate and assayed after 24 hours with a LIVE/DEAD Viability/Cytotoxicity kit (Thermo Fisher Scientific). Fluorescence images were acquired using GFP and TXRED filter cubes on an Olympus IX81 microscope with a 10× objective (0.25 NA; Zyla 4.2 sCMOS camera) and quantified manually in ImageJ.

Cell proliferation analysis by assaying Ki-67 protein was done as follows: MCF10A cells were seeded at an initial density of 2600 cells/cm2, cultured for 48 hours after seeding, and then fixed using 4% paraformaldehyde at room temperature for 30 min. Samples were incubated with Ki-67 primary antibody, followed by Alexa Fluor–conjugated secondary antibody. Nuclei were stained with Hoechst. The number of Ki-67–positive cells and total number of cells were counted manually with ImageJ.

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