Experimental design
Gel fabrication
Gel functionalization with fibronectin
Gel functionalization with TAMRA-RGD peptide
Silicone breast implant–derived materials
Mechanical characterization
Interfacial rheology measurements
Contact angle measurements
Steel ball indentation measurements
Gel preparation with Span85 surfactant treatment for cellular assays
Cell lines and culture
Cell morphology and spreading assays
Cell proliferation assays
Gene expression analysis
Isolation and culture of PBMCs
Preparation of a collagen master mix for 3D collagen I gels
PBMC-silicone interaction assay
PBMC—silicone droplets interaction assay
Immunofluorescence imaging
Hertz contact model with surface tension
Statistics
The following antibodies were used: monoclonal FAK (13009S, Cell Signaling Technology); FAKpY397 (8556S, Cell Signaling Technology); YAP (sc-101199, Santa Cruz Biotechnology); Ki-67 (9129S, Cell Signaling Technology). Alexa Fluor 647 anti-MHCII antibody (cz11, clone 130.8E8D9) was a gift of D. Antczak, Cornell University, Ithaca, NY, USA. The FAK inhibitor was PF-573228 (5 μM; 14924, Cayman Chemical). The Src inhibitor was dasatinib (0.1 μM; 11498, Cayman Chemical).