In vitro transcription assays were performed using a MEGAscript kit (Life Technologies, USA), according to the manufacturer’s instructions. PTPRE-AS1 RNAs were labeled by desthiobiotinylation, using the Pierce RNA 3′End Desthiobiotinylation Kit (Thermo Scientific). Then, RNA pull-down assays were performed using the Magnetic RNA-Protein Pull-Down Kit (Thermo Scientific).

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