RIP was performed using the EZ-Magna RIP Kit (Millipore). Cells (1 × 107) were lysed with RIP lysis buffer. Cell extracts were coimmunoprecipitated with anti-WDR5, EZH2, LSD1, SETDB1 (Cell Signaling Technology), or DNMT1 (Abcam), and the retrieved RNA was subjected to RT-qPCR analysis. For RT-qPCR analysis, U1 was used as a nonspecific control target.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.