BMDMs were stimulated with LPS (200 ng/ml) or IL-4 (20 ng/ml) for 3 and 24 hours, respectively. Total RNA was extracted and purified using the miRNeasy Mini Kit (Qiagen). The lncRNA mRNA Mouse Gene Expression Microarray V 6.0 (Agilent Technologies) was used to investigate differentially expressed lncRNAs in BMDMs after IL-4 or LPS stimulation. Microarray slides were sequenced on an Agilent Microarray Scanner, and the raw data were normalized by Quantile algorithm, GeneSpring Software 12.6.1 (Agilent Technologies) at Shanghai Biotechnology Corporation. To comprehensively analyze the microarray data, filtering criteria were applied (≥2-fold change, P < 0.05).

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