BMDMs were stimulated with LPS (200 ng/ml) or IL-4 (20 ng/ml) for 3 and 24 hours, respectively. Total RNA was extracted and purified using the miRNeasy Mini Kit (Qiagen). The lncRNA mRNA Mouse Gene Expression Microarray V 6.0 (Agilent Technologies) was used to investigate differentially expressed lncRNAs in BMDMs after IL-4 or LPS stimulation. Microarray slides were sequenced on an Agilent Microarray Scanner, and the raw data were normalized by Quantile algorithm, GeneSpring Software 12.6.1 (Agilent Technologies) at Shanghai Biotechnology Corporation. To comprehensively analyze the microarray data, filtering criteria were applied (≥2-fold change, P < 0.05).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.