PTPRE-AS1–null (PTPRE-AS1-KO) mice were generated using the CRISPR-Cas9 system at the Shanghai Model Organisms Center Inc. Briefly, the CRISPR-Cas9 system was applied in zygotes from C57BL/6 mice, and mutations were introduced by nonhomologous recombination repair, resulting in deletion of exons 2 and 3, and loss of function of lncRNA PTPRE-AS1. PTPRE-AS1–null mouse genotypes were confirmed by DNA sequencing. Primers used to identify genetically modified mice are listed in table S2. All mice were housed, bred, and maintained under specific pathogen–free conditions. All experiments complied with the relevant laws and institutional guidelines, as overseen by the Animal Studies Committee of the Children’s Hospital of Fudan University.

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