For the infection procedure, P. aeruginosa AA43 strain isolated from a patient with CF before death was embedded in agar beads. Mice were anesthetized to implant into the lung an inoculum of 50 μl of agar bead suspension, embedding 1.5 × 106 CFU of AA43 strain.

Mice were treated with KB-R7943 (300 μg/kg) or vehicle via aerosol administration by Penn-Century MicroSprayer Aerosolizer every 12 hours starting 1 hour before infection. Two days after infection (1 hour after last treatment), mice were sacrificed to collect BALF and lungs for histopathological analysis. A detailed description of all the procedures used is reported in Supplementary Materials and Methods section.

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