To assess rejection, hindlimbs were monitored daily and scored for rejection (appearance grading) based on physical examination (39). Limbs were given a daily score using the following scale: grade 0 (no rejection), grade I (edema), grade II (erythema and edema), grade III (epidermolysis), and grade IV (necrosis and “mummification”). Grafts were considered rejected when displaying signs of progressive grade III rejection.

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