Cells were seeded at a density of 750 cells per well in white opaque 384-well plates using a multidrop microplate dispenser. The following day, compounds were added and incubated for 72 hours, followed by addition of CellTiter-Glo (10 μl per well) (Promega), and luminescence was measured using an Infinite M200 PRO plate reader (Tecan Group Ltd.). Cell viability was normalized to DMSO controls.

Cell viability with catalase addition. The assay was run similarly to the CellTiter-Glo assay. However, catalase (10 μl per well) (C30, Sigma-Aldrich) to a final concentration of 100 U/ml was added to cells 4 hours before addition of the compounds.

Cell viability with selenite addition. The assay was run exactly as the CellTiter-Glo assay. However, cells were grown at least 72 hours with the addition of 100 nM sodium selenite in their respective growth medium before cell seeding of the experiment.

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