Cells were fixed with 4% paraformaldehyde for 30 min and blocked in 0.1% bovine serum albumin (BSA) for 30 min at room temperature. Cells were stained with anti-Cdh1 (Cell Signaling, 3195, 1:200) and then secondary antibody goat anti-rabbit DyLight 488 (Abcam, ab96883, 1:200). Next, cells were analyzed with a BD Accuri C6 Plus flow cytometer.

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