4TM (H6278, Sigma-Aldrich Chemie N.V., Netherlands) was prepared as described previously (21). First, 10 mg of 4TM was first dissolved in 200 μl of 100% dimethyl sulfoxide (DMSO) (D8418, Sigma-Aldrich Chemie N.V., Netherlands). This stock solution was stepwise-diluted in 1900 μl of saline containing 2%Tween 80 (P1754, Sigma-Aldrich Chemie N.V., Netherlands) and then once more in the same volume of saline. This resulted in a final solution of 4TM (2.5 mg/ml), 5% DMSO, and 1% Tween 80 in saline. Animals received 4TM [25 mg/kg, intraperitoneally (ip)] 2 hours after a tag session (see below).

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