Two adult male New Zealand white rabbits bearing VX2 tumor in the erector spinae muscle were provided by the Molecular imaging and functional imaging laboratory of Southeast University (Nanjing, China). A total of 76 healthy adult male New Zealand white rabbits (age, 12 weeks; weight, 2.5 to 3.0 kg) purchased from DaShuo experimental animal Co. Ltd. (Chengdu, China) received surgeries, including 44 animals to establish VX2 tumor model in the erector spinae muscles to investigate the antitumor efficacy of n-HA, μ-HA, and n-TiO2 particles and 32 animals to establish femur defect tumor model. Rabbits were individually caged and kept in a controlled temperature at 25 ± 2°C with food and water provided ad libitum.

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