A549 cell culture. Two cell lines were used in this work: adenocarcinomic human lung epithelial cell line (A549) and human cellosaurus cell line (BJ). The A549 cells were grown in DMEM with 10% FBS. The BJ cells were grown in MEM with 10% FBS and 1× nonessential amino acids. All cells were cultured on 35-mm MatTek dishes (MatTek Corp.) at 37°C and 5% CO2. Confluency of about 60% was reached for all experiments.

Electron microscopy sample preparation and TEM/STEM data collection. For electron microscopy experiment, all cells were prepared by the ChromEM staining protocol and embedded in Durcupan resin (EMS) (27). After curing, 40-nm-thin sections were made and deposited onto copper 200-mesh grid with carbon/formvar film (EMS). The grids were plasma-cleaned by a plasma cleaner (easiGlow, TED PELLA) before use. An HT7700 (HITACHI) transmission electron microscopy (TEM) was used to record TEM images of cell sections at 80 kV with a pixel size of 2.5 nm.

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