Study design
Experimental models
Primary cell culture
Virus production, infection, and selection
Antibodies
Western blot analysis
Immunoprecipitation assay
Phosphorylation assay for Flt1
Histology and immunostaining
Image acquisition
FACS isolation of epithelial cells for RNA sequencing
RNA extraction and real-time RT-PCR
List of primers
ATAC-seq and library preparation
ATAC-seq analysis
Motif analysis
GO analysis
Quantification and statistical analysis
Mice were treated by Nrp1-blocking antibodies: anti-Nrp1a, which blocks binding of semaphorins, and anti-Nrp1b, which blocks binding of Vegfa (Genentech, 10 mg/kg) by intraperitoneal injection, two times per week during 15 days as described previously for tumor models (14, 26). The antibodies were generated and provided by Genentech.