Cells were serum starved for 12 hours and stimulated either by rVEGFA (50 ng/ml; R&D, catalog number 293 CF) or by hPLGF (human placental growth factor) (50 ng/ml; R&D, catalog number 264-PGB). We used as control condition nonstimulated transfected hFlt1-HEK293 cells as previously described (13).

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