The following primary antibodies were used: anti-Ki67 (rabbit, 1:200, Abcam, catalog number ab15580), anti-K14 (rabbit, 1:1.000, Thermo Fisher Scientific, catalog number PA5-28002), anti-Nrp1 (goat, 1:100, R&D, catalog number AF 566), anti-CD31 (rat, 1:200, BD Biosciences, Clone MEC13.3, catalog number 550274), anti-CD45 (rat, 1:500, BD Biosciences, clone 30F11, catalog number 12-0451/553081), anti-Fosl1 (mouse, 1:500,Santa Cruz, clone C12, catalog number sc-28310), CD4 (rat, 1:100, BD Biosciences, clone RM4-5, catalog number 565650), CD8 (rabbit, 1:100, Abcam, catalog number ab203035), CD19 (rat, 1:100, BD Biosciences, clone 1D3, catalog number 561737), Ly6G (rat, 1:100, BioLegend, clone 1A8, catalog number 177603), F4/80 (rat, 1:500, Serotec, clone A3-1, catalog number MCA497 GA), TLR4 (rabbit, 1:100, Abcam, ab22048), and ISM1 (rabbit, 1:100, Abcam ab103338). The following secondary antibodies were used: anti-rabbit, anti-rat, and anti-chicken conjugated to Alexa Fluor 488 (1:400, Molecular Probes), to Rhodamine Red-X, or to Cy5 (1:400, Jackson ImmunoResearch).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.